ZASTOSOWANIE METODY REAL-TIME PCR I SYSTEMU LIGHTCYCLER® DO WYKRYWANIA DNA LUDZKIEGO HERPESWIRUSA TYPU 7 (HHV-7)
[APPLICATION OF REAL-TIME PCR AND LIGHTCYCLER® SYSTEM FOR INVESTIGATING THE PRESENCE OF HUMAN HERPESVIRUS 7 DNA]

Streszczenie.

Celem pracy było zaprojektowanie, optymalizacja warunków reakcji oraz zbadanie użyteczności metody real-time PCR z wykorzystaniem sond typu TaqMan do diagnostyki zakażeń ludzkim herpeswirusem typu 7, zwłaszcza u pacjentów z oddziałów onkohematologicznych.

Abstract.

Human herpesvirus 7 (HHV-7) is a β-herpesvirus widely spread within a population and has been recognized as a potential pathogen in immunocompromised hosts. The goal of the study was development of real-time PCR assay for detection of human herpesvirus 7 DNA in clinical samples,
using primers targeting a conserved region of the viral DNA major capside proteine gene and a specific
TaqMan hydrolyzing probe. Sixty four plasma samples taken from a group of adult recipients of allogeneic HSCT, during detectable CMV viremia or neutropenic fever, were tested for the presence of viral DNA in the LightCycler® system with method described above. HHV-7 DNA was detected in 40 specimens (62,5%). The conclusion is that developed TaqMan-based probes real-time PCR test
is very reliable and valuable tool for detection of HHV-7 viremia in plasma samples. The high level of sensitivity and accuracy provided by the LightCycler® instrument is favorable for the use of this method in the detection of human herpesvirus 7 DNA in clinical specimens.