PDF

Medycyna Doświadczalna i Mikrobiologia 2008, 60(2): 131-138

WPŁYW MATERIAŁÓW UWALNIAJĄCYCH FLUOR NA SKŁAD PŁYTKI NAZĘBNEJ
[INFLUENCE OF THE FLUORIDE RELEASING DENTAL MATERIALS ON THE BACTERIAL FLORA OF DENTAL PLAQUE]

M. Płuciennik, D. Sakowska, Z. Krzemiński, D. Piątowska

Streszczenie.

Oceniano wpływ trzech materiałów uwalniających in vivo fluor na skład bakteryjnej flory płytki nazębnej po 10 tygodniach od chwili wprowadzenia ich do ubytków kl V.

Abstract.

The assessment of influence of silver-free, fluor releasing dental materials on dental plaque bacteria quantity. 17 patients were included into the study. 51 restorations were placed following manufacturers recommendations. Following materials were used: conventional glassionomer Ketac-Molar ESPE, resin modified glassionomer Fuji II LC GC and fluor containing composite Charisma Heraeus Kulzer Class V restorations were placed in following teeth of upper and lower jaw: canines, first bicuspids,
second bicuspids. Sound enamel was a control. After 10 weeks the 72 hours old dental plaque was collected from surface of restorations and control using sterile probe. Total amount of 68 dental plaques were investigated. Each plaque was placed on scaled and sterile aluminum foil. The moist weight of dental plaque was scaled. Dental plaque was moved into 7 ml 0,85% NaCl solution reduced
by cystein chlorine hydrogen and disintegrated by ultrasounds (power:100 Watt, wave amplitude:
5μm). The suspension of dental plaque was serially diluted from 10 –4 to 10 –5 in sterile 0,85% NaCl
solution, and seeded with amount of 0,1 ml on appropriate base. In dental plaque trials the amount of
cariogenic bacteria was calculated - Streptococcus mutans, Streptococcus, Lactobacillus, Veillonella
and Neisseria, and also total amount of aerobic and anaerobic bacteria was measured. Microbiologic
studies were performed in Institute of Microbiology, Medical University, Łódź. Statistical analysis of
collected data was accomplished. In 72 hours old dental plaques collected from the surfaces of Ketac -Molar, Fuji II LC, Charisma after 10 weeks since being placed into the class V cavity, results show no statistically significant differences in the amount of Streptococcus mutans, Streptococcus spp., Lactobacillus spp., Veillonella
spp., Neisseria spp, in total amount of aerobic and anaerobic bacteria and in the quantity proportion
of Streptococcus mutans versus Streptococcus spp. in comparison with control trail. Results show no statistically significant differences in the amount of listed above bacteria and in the proportion of Streptococcus mutans versus Streptococcus spp. in 72 hours old dental plaques collected from surfaces of investigated restorative materials.

Liczba pobrań: 2104