Ocena wrażliwości szczepów bezwzględnych beztlenowców pochodzących z różnych źródeł na fluorochinolony oraz inne leki stosowane w lecznictwie szpitalnym
[Assessment of susceptibility of strictly anaerobic bacteria originated from different sources to fluoroquinolones and other antimicrobial drugs]

Streszczenie

Na świecie obserwuje się wzrost oporności bakterii bezwzględnie beztle­nowych na leki stosowane w lecznictwie szpitalnym, w tym na nowe fluo­rochinolony. W pracy potwierdzono wzrastającą oporność bezwględnych beztlenowców z grupy Bacteroides fragilis (BFG) oraz gatunku Clostridium difficile na fluorochinolony oraz inne leki stosowane w leczeniu zakażeń mieszanych, w których uczestniczą beztlenowce. Odnotowano obniżoną wrażliwość na wankomycynę u 13% szczepów C. difficile.  

Abstract

Introduction. During the past 20 years, several studies at a national level in different co­untries followed resistance trends for Bacteroides sp. and Clostridium difficile. This study analysed antimicrobial susceptibility 73 anaerobic bacteria strains of Bacteroides fragilis group (BFG) and C. difficile to fluoroquinolones and other antimicrobial drugs. Methods. The strictly anaerobes strains isolated in different hospitals were sent to the Department of Medical Microbiology, Medical Uniwersity of Warsaw, where species de­termination was carried out with the API20 ANA (bioMerieux SA, Marcy-l’Etoile, France) system. Susceptibility to antimicrobials was determined using E-test. Results. The rates of high resistance to ciprofloxacin and moxifloxacin of BFG was re­spectively 84% and 31% and among of C. difficile strains respectively 92% and 36%). The percentage of BFG strains resistant to erythromycin and clindamycin were respectively 84% and 46%. The percentage of C. difficile strains resistant to erythromycin and clindamycin was 52%. Reduced level of susceptibility of BFG strains to amoxicillin/clavulanic acid (8%) was confirmed. Resistance to cefoxitin was 16% of BFG strains. All tested strains as well as BFG and C. difficile were susceptible to metronidazole. Was observed reduced level (EUCAST) of susceptibility of C. difficile strains to vancomycin (13%). Conclusions. Increasing resistance to various antimicrobial agents is a significant problem in Poland. This demonstrate the need to continue with antibiotic resistance testing and surveys in anaerobic bacteria.