OCENA STOPNIA WRAŻLIWOŚCI NA AMINOGLIKOZYDY SZCZEPÓW BAKTERYJNYCH IZOLOWANYCH OD CHORYCH Z ZAKAŻENIAMI UKŁADOWYMI I UOGÓLNIONYMI
[ESTIMATION OF LEVEL OF AMINOGLYCOSIDES SUSCEPTIBILITY OF BACTERIAL STRAINS ISOLATED FROM PATIENTS WITH SYSTEMIC AND GENERAL INFECTIONS]

Streszczenie.

Zbadano wrażliwość na antybiotyki aminoglikozydowe, z określeniem wartości MIC, 200 wybranych szczepów bakteryjnych izolowanych w okresie od 1.01.2007 do 30.04.2008 od chorych hospitalizowanych w różnych oddziałach klinik wrocławskich. Wśród badanych szczepów było 96 pałeczek z rodziny Enterobacteriaceae, w tym 19 ESBL(+), 74 pałeczki niefermentujące, w tym 30 szczepów opornych na karbapenemy i 30 szczepów S.aureus, w tym 2 MRSA.

Abstract.

Susceptibility tests to aminoglycosides of 200 bacterial strains isolated in period 1.01.2007- 30.04.2008 from hospitalized patients in different Wroclaw clinics were performed. Among tested strains were 96 Gram-negative rods belonging to the Enterobacteriaceae family, 74 non-fermentative rods and 30 strains of Staphylococcus aureus. The results of antibiotic susceptibility determined by serial antibiotic dilution in solid medium indicated that among Enterobacteriaceae the most susceptible
to aminoglycosides were E.coli ( 90-95%) and least susceptible Enterobacter spp., Citrobacter spp. and
Serratia spp ( 62-76%). Among non-fermentative rods, Pseudomonas aeruginosa was more susceptible
than Acinetobacter spp. 97% strains of Staphylococcus aureus were susceptible to all aminoglycosides.
Enterobacteriaceae strains isolated from adults were more resistant to aminoglycosides than isolated from children and non-fermentative rods isolated from adults more susceptible to netilmicin. Among S.aureus only one strains was MRSA and aminoglycoside resistant. Twenty two of 96 examined strains belonging to Enterobacteriaceae and 46 of 74 examined non-fermentative rods were resistant at least to one aminoglycoside. Predominant resistance patterns were as follow: simultaneous resistance to
all aminoglycosides and resistance to gentamicin and netilmicin with susceptible to amikacin. High level resistance ( MIC ≥256μg/ml) was found in 23 gentamicin resistant strains ( 12 rods of Enterobacteriaceae
and 11 non-fermentative rods), in 33 netilmicin resistant strains ( respectively 10 and 21 strains) and in 14 amikacin resistant Gram-negative rods ( respectively 9 and 5 strains). Among 96 rods of Enterobacteriaceae family 19 ESBL (+) strains were found. High level of resistance ( MIC ≥256μg/ml) was found in 10 ESBL (+) strains.