OCENA PRZYDATNOŚCI TESTU PREMI®TEST SALMONELLA DO IDENTYFIKACJI PAŁECZEK SALMONELLA NIETYPUJĄCYCH SIĘ METODAMI KLASYCZNYMI
[EVALUATION OF PREMI®TEST SALMONELLA KIT FOR IDENTIFICATION OF NOTTYPABLE BY CONVENTIONAL METHODS SALMONELLA]

Streszczenie

Oceniono przydatność komercyjnego testu Premi®Test Salmonella do identyfikacji nietypujących się szczepów pałeczek Salmonella. Spośród 37 przebadanych szczepów, ocenianym testem w pełni zidentyfikowano 21 (56%) szczepów, identyfikacja 5 (14%) szczepów wymagała dodatkowego potwierdzenia, niepełną identyfikację uzyskano w przypadku 5 (14%) szczepów, natomiast 6 (16%) szczepów nie udało się zidentyfikować. Uzyskane wyniki świadczą o przydatności testu Premi®Test Salmonella do określania serotypu pałeczek Salmonella, w przypadku gdy jest to niemożliwe tradycyjnymi metodami.

Abstract

Despite the downward trend, Salmonella is still one of the most important bacterial intestinal infections agents. For example, in 2007 y. in Poland over 14 thousands human salmonelosis cases were notified, in 2008 y. - over 10 thousands. Among all Salmonella isolated from human source, most common (more then 80%) are two serotypes – S. Enteritidis and S. Typhimurium, but every year the number of non-typable (because of the lack I or II phase flagellar antigens, autoagglutinative or
non-motility properties) Salmonella is growing. This work describes the results of the evaluation of commercially available kit Premi®Test Salmonella (DSM, Netherlands), which uses the microarray hybridization in ArrayTube, for serological identification of non-typable Salmonella strains. All 37 researched strains were submitted to the Department of Bacteriology in 2007-2008 y, were reidentified according to standard operating procedures and serotyped by slide agglutination for somatic and
flagellar antigens if it was possible. All strains were tested using the Premi®Test Salmonella Kit, according to manufacturer instructions. In 21 cases (56%) full identification were achieved, in 5 cases (14%) additional tests were required for precise identification (Salmonella Choleraesuis or Paratyphi C, what was detailed using examination of additional biochemical features), 5 strains (14%) achieved an incomplete identification (three of them – S. diarizonae were confirmed in National Reference
Laboratory for Salmonella) and 6 cases (16% ) were not identified at all. The total number of recognized strains is 30 (81%). The results of present studies show, that Premi®Test Salmonella Kit is useful for non-typable Salmonella identification.