Ocena przydatności testu API ZYM i podłoża RPMI wzbogaconego w Tween 40 do wykrywania lipolitycznych enzymów Candida sp.
[Evaluation of the API ZYM system and RPMI agar plates supplemented with Tween 40 for detection of lipolytic enzymes of Candida spp.]

Streszczenie

Ocenę przydatności testów API ZYM i podłoża RPMI wzbogaconego w Tween 40 do wykrywania enzymów lipolitycznych przeprowadzono u 40 szczepów Candida sp. izolowanych z krwi i moczu. Stosując podłoże RPMI wzbogacone w Tween 40 częściej wykrywano wytwarzanie lipazy u badanych drożdży niż za pomocą testów API ZYM.

Abstract

Lipolytic activity of 40 strains of Candida spp. was tested on API ZYM system and on RPMI agar plates supplemented with 1% Tween 40. Lipolytic activity was indicated by opaque zones around the inoculum cylindrical holes were punched in the medium. Clearing of the medium around the bacterial colonies indicated that an isolate produce lipase. Only 4 (21.1%) strains of C. albicans, and 3 (14.1%) strains of non-C. albicans which hydrolyzed 2-naftylomirystylan by use of the API ZYM
system was observed. In contrast, 16 (78.9%) strains of C. albicans and 17 (80.7%) strains of non-C.
albicans produced lipases on the agar plate using RPMI agar plates supplemented with 1.0% Tween
40. Determination of lipase activities with the API ZYM system were in no agreement with lipase tests in RPMI supplemented with Tween 40. Our study verify greater usefulness of RPMI supplemented with Tween 40 for detection of lipolytic enzymes of Candida species in comparison to the API ZYM.