OCENA ODCZYNEM WESTERN- IMMUNOBLOTTING ANTYGENOWYCH WŁAŚCIWOŚCI BIAŁEK CAMPYLOBACTER JEJUNI I CAMPYLOBACTER COLI
[EVALUATION OF ANTIGENIC PROPERTIES OF CAMPYLOBACTER JEJUNI AND CAMPYLOBACTER COLI PROTEINS IN A WESTERN – IMMUNOBLOT]

Streszczenie.

Oceniono właściwości antygenowe wybranych białek pałeczek z rodzaju Campylobacter. Badane szczepy C. jejuni jak i C. coli charakteryzowały się wysokim podobieństwem profilu białkowego i podobną reaktywnością antygenową. Najbardziej swoistymi w odczynie western-immunoblotting były białka o masie cząsteczkowej 92, 62, 56, 52, 45-43, 29 kDa, które mogłyby być wykorzystane jako wysoce swoiste antygeny w serodiagnostyce kampylobakteriozy.

Abstract.

Campylobacter jejuni and Campylobacter coli are the most common bacterial cause for acute diarrheal illnesses in developed countries. The aim of this study was to evaluate the antigenic properties of Campylobacter jejuni and Campylobacter coli proteins in western-blot assay. Whole – cell components of Campulobacter jejuni and Campylobacter coli were separated by sodium dodecyl sulfate – polyacrylamide gel electroforesis. Using this method we detected in all seven C. jejuni strains 21
peptides migrating between 180-29 kDa. All three C.coli strains had a 17 bands migrating with the same molecular weight range. Proteins were transferred electrophoretically to nitrocellulose paper for
immunoblotting experiments. The 74 kDa protein reacted strongly in all classes of immmunoglobulin with all tested human serum samples. We observed that this protein reacted also with human immunoglobulins for Salmonella and Yersinia sp. This cross - reaction observed for this protein could give false positive results in routine diagnosis of C.jejuni infections. The proteins with molecular weight of: 92, 62, 56, 52, 45-43, 29 kDa were most recognized in the 20 human serum samples. The other proteins of C.jejuni and C. coli, particularly in the 68-50 kDa and 45-31 kDa regions, were recognized
occasionally and the response to these in reconvalescent sera was usually weak. The result of this study showed that the proteins with molecular weight: 92, 62, 56, 52, 45-43 and 29 kDa can be use in routine serological diagnostic of campylobacteriosis.