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Medycyna Doświadczalna i Mikrobiologia 2019, 71(1): 51-57

Nowe gatunki w obrębie kompleksów Candida parapsilosis i Candida glabrata
[New species within Candida parapsilosis and Candida glabrata]

Dariusz Domański, Magdalena Anna Sikora, Robert Tomasz Kuthan, Ewa Augustynowicz-Kopeć, Ewa Swoboda-Kopeć

STRESZCZENIE

Candida parapsilosis i Candida glabrata to kolejne, po Candida albicans, drożdżaki tworzące kompleksy kryptogatunków. Pomimo, że gatunki te zostały opisane ponad dekadę temu, nadal wiedza na ich temat jest ograniczona.
Celem pracy była identyfikacja gatunkowa szczepów klinicznych w obrębie kompleksów C. glabrata i C. parapsilosis. W wyniku przeprowadzonej analizy molekularnej wykryto 24 izolaty Candida nivariensis oraz 4 szczepy C. metapsilosis i 9 szczepów C. orthopsilosis.

ABSTRACT

Introduction: Candida parapsilosis and Candida glabrata are another yeasts that form complexes of crypospecies. Although these species have been described more than a decade ago, knowledge about them is still limited. The reason for this is the large phenotypic similarity that unables them from being differentiated by classical diagnostic methods. The aim of the study was to identify species of clinical strains within C. glabrata and C. parapsilosis complexes.
Material and methods: Standard PCR-RFLP of the secondary alcohol dehydrogenase gene (SADH) with BanI restriction enzyme served to determine species affiliation within the C. parapsilosis complex. The internal transcribed spacer was used to confirm the identification of C. glabrata sensu stricto. The D1/D2 domain of the 26S rDNA gene was sequenced in order to identify C. nivariensis and C. bracarensis strains.
Results: As a result of the molecular analysis, 24 Candida nivariensis isolates and 4 C. metapsilosis strains and 9 C. orthopsilosis strains were detected.
Conclusions: Prevalence of new cryptic species was relatively low.

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