Lekowrażliwość a zdolność tworzenia biofilmu przez Candida sp.
[Susceptibility to antifungal agents of Candida sp. and biofilm formation]

Streszczenie

Ocenę lekowrażliwości przeprowadzono u 50 szczepów Candida sp. izolowanych z krwi i moczu od chorych hospitalizowanych, wykorzystując testy ATB FUNGUS 2 INT (bioMérieux) oraz E-testy z worikonazolem, posakonazolem, kaspofunginą i anidulafunginą (AB BIODISK). Zdolność tworzenia biofilmu oceniano metodą redukcji TTC na powierzchni 5 biomateriałów: lateksie silikonowanym, silikonie, polichlorku winylu, nylonu i polipropylenu. Stwierdzono, że szczepy oporne na antymikotyki częściej tworzyły biofilm na powierzchni badanych biomateriałów.

Abstract

In recent years the increase in frequency of fungal infections with Candida sp. was noticed. These
infections are connected with ability of Candida sp. to form biofilm on surfaces of biomaterials used in medicine. Furthermore fungal infections make serious therapeutic problems because of biofilm resistance to antifungal agents actually. The aim of the study was to evaluate the susceptibility to antifungal agents of Candida sp. and their ability to form biofilm on different biomaterials. 50 strains of Candida sp. isolated from patients of University Hospital No. 1 of dr A. Jurasz in Bydgoszcz were examined. API Candida (bioMérieux) tests were used to identify Candida sp. strains. The susceptibility of the yeast strains to antifungal agents was evaluated by ATB FUNGUS 2 INT (bioMérieux) tests. The susceptibility of examined strains to voriconazole, posaconazole, caspofungin and anidulafungin was assessed by means of Etests (AB BIODISK) method employing drug concentrations from 0,002 to 32 μg/ml. All analysed strains were susceptible to amphotericin B and caspofungin. Biofilm formation on different biomaterials (silicon, latex, polychloride vinyl, polypropylene, nylon) was measured after 72 hour incubation at 37˚C. All examined yeasts formed biofilm on all analysed biomaterials. The highest number of strains formed biofilm on surface of polychloride vinyl: 23 (92,0%) by C. albicans strains and 24 (96,0%) Candida non-albicans strains. The lowest number of the strains formed biofilm on the surface of nylon: 12 (48,0%) of C. albicans strains and 9 (36,0%) of Candida non-albicans strains. The studied strains resistant to azoles and anidulafungin display stronger ability to form biofilm on surfaces of all analysed biomaterials.