GENETYCZNA CHARAKTERYSTYKA SZCZEPÓW ACINETOBACTER BAUMANNII ORAZ PSEUDOMONAS AERUGINOSA IZOLOWANYCH OD CHORYCH Z ZAPALENIEM PŁUC HOSPITALIZOWANYCH W ODDZIALE INTENSYWNEJ TERAPII
[THE GENETIC DESCRIPTION OF ACINETOBACTER BAUMANNII AND PSEUDOMONAS AERUGINOSA STRAINS ISOLATED FROM PATIENTS WITH HOSPITAL ACQUIRED PNEUMONIA HOSPITALIZED IN INTENSIVE CARE UNIT]

Streszczenie

Przedmiot badań stanowiły 33 szczepy Acinetobacter baumannii oraz 31 szczepów Pseudomonas aeruginosa izolowanych z aspiratów tchawicznych, pobranych od 19 chorych, z potwierdzonym mikrobiologicznie szpitalnym zapaleniem płuc, hospitalizowanych w Oddziale Anestezjologii i Intensywnej Terapii Wojewódzkiego Szpitala Specjalistycznego im. L. Rydygiera w Krakowie. Izolaty genotypowano z wykorzystaniem metody RAPD-PCR, przy użyciu primerów 208, 272, ERIC-2 oraz PAL-2. Obserwowano zróżnicowanie w podobieństwie genetycznym analizowanych populacji szczepów niefermentujących pałeczek Gram-ujemnych.

Abstract

The aim of study was to recognize the epidemiology of hospital acquired pneumonia on the base of genotypes of Acinetobacer baumannii and Pseudomonas aeruginosa strains. Gram negative- non fermenting bacilli are the most frequent aetiologic agent of hospital acquired pneumonia among patients from Anesthesiology and Intensive Care Unit in the Rydygiers hospital in Cracow . In the following research RAPD- PCR method was applied and there 272, 208, ERIC- 2 and PAL- 2 primers
were used. The investigation was conducted among 33 Acinetobacter baumannii and 31 Pseudomonas
aeruginosa strains which were isolated from endotracheal aspirates (ETA). ETA were taken from 19 patients with microbiologically confirmed pneumonia. The result of our study shows that most A. baumannii strains came from hospital habitat. In the investigated group of A. baumannii strains, 31 belonged to 3 clonal groups and probably came from two bacteria subpopulations which were present in ICU from a long time. There were only two isolates which had got the posthospital origin. Moreover, the assay of genetic similarity between A. baumannii strains, isolated from individual patients, showed that only in two patients were observed strains with different genetic profiles. Isolates which came from 8 patients had got the same pattern of bands. There were many genetic differences
between investigated P. aeruginosa strains. In the group of 31 isolates, which were investigated by using 208, 272, ERIC- 2 and PAL- 2 primers, recognized respectively 10, 10, 13 and 8 of genetic profiles. All isolates of P. aeruginosa probably came from a few subpopulations of hospital strain which has undergone evolutionary divergence phenomenon in time as a result of changing condition of hospital environment and application of antibiotics. The assay of genotype of P. aeruginosa strains which were repeatedly isolated from individual patients showed that only from two patients strains
with 100% genetic homology were isolated.