Fenotypowe cechy pałeczek Pseudomonas putida izolowanych z różnych środowisk
[Phenotypic characteristics of Pseudomonas putida isolated from different environments]

Streszczenie.

Badaniami objęto 34 szczepy P. putida, z których 27 izolowano z gleby zanieczyszczonej pochodnymi ropy naftowej, 3 z biopreparatów stosowanych w procesach biodegradacji zanieczyszczeń oraz 4 z ran i owrzodzeń. Podjęto próbę wewnątrzgatunkowego różnicowania szczepów P. putida wykorzystując ich aktywność biochemiczną, wzory lekowrażliwości i określając zymotypy. Stwierdzono przydatność tych metod w diagnostyce mikrobiologicznej pałeczek P. putida.

Abstract.

Various typing methods have been suggested to differentiate isolates of P. putida species with the aim of developing epidemiological tools. 34 P. putida strains were isolated from the samples of crude oil and oil derivates contaminated soil (n = 27), biopreparates used for biodegradation of soil contamination (n = 3) and hospital materials ( n = 4). The biochemical typing was assessed using ID32GN tests (bio-Merieux). The strains were grouped into 11 biotypes. Antibiotic-containing discs
were used for routine antibiograms by disc diffusion assay. The strains were most resistant to cefoperazone
and ticarcillin (29,4% and 26,5% of strains, respectively). The intracellular esterases of P. putida were separated by polyacrylamid gel electrophoresis and stained with Fast Blue using alpha-naphthyl acetate, beta-naphthyl propionate and indoxyl acetate as substrates. On the basis of the indicators dye migration the electrophoresis process was observed. By calculating the RF (retention factor) the distribution of stripes was obtained with great accuracy. The use of biochemical tests, sensitivity tests and zymotyping provides the possibility to inter- and intraspecies differentiation of P. putida.