ANALIZA WRAŻLIWOŚCI IN VITRO KLINICZNYCH IZOLATÓW HERPESWIRUSA CZŁOWIEKA TYPU 1 (HIV-1) NA ACYKLOWIR I CIDOFOWIR
[IN VITRO ACYCLOVIR AND CIDOFOVIR SUSCEPTIBILITIES OF HUMAN HERPESVIRUS TYPE 1 CLINICAL ISOLATES]

Streszczenie.

Wirus opryszczki jest ludzkim patogenem odpowiedzialnym za zakażenia o łagodnym jak i ostrym przebiegu. Intensywne używanie leków przeciwwirusowych, w szczególności acyklowiru prowadzi do powstawania i selekcji szczepów opornych. W niniejszej pracy określono i porównano wrażliwość klinicznych izolatów wirusa opryszczki typu 1 na acyklowir i cidofowir.

Abstract.

Infections with human herpesviruses types 1 and 2 (HHV-1 and HHV-2) are common worldwide and cause a wide range of signs and symptoms. Antiviral drugs, in particular aciclovir are used in therapy of herpetic infections. The aim of the study was determination of susceptibilities of HHV-1 isolates (n+46) for antiviral drugs (acyclovir and cidofovir) in vitro. Swabs taken from different lesions
were used for infection of Vero cells and cythopathic effect was observed. Viruses from cell cultures with positive CPE were later identified with in-house PCR and efficacy of acyclovir and cidofovir in HHV-l infected Vero cell monolayer cultures was tested by the yield reduction assay. Obtained data indicate, that aciclovir ID50 average value for HHV-1 clinical isolates was 0,74 μg/ml – the value about 10% greater then described in literature. Similarly in vitro analysis of sensitivity of viruses for
cidofovir, shows that concentrating is over ten-fold higher in comparison for aciclovir.