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Medycyna Doświadczalna i Mikrobiologia 2008, 60(2): 111-119

WRAŻLIWOŚĆ NA ANTYBIOTYKI ORAZ WYSTĘPOWANIE BETA-LAKTAMAZ TYPU IBL, ESBL I MBL U SZCZEPÓW PSEUDOMONAS AERUGINOSA
[ANTIBIOTIC SUSCEPTIBILITY AND OCCURRENCE OF ESBL, IBL AND MBL IN PSEUDOMONAS AERUGINOSA STRAINS]

K. Wolska, A. Jakubczak, A. Soszyńska

Streszczenie.

Wykazano, że ponad 80% szczepów P. aeruginosa izolowanych od hospitalizowanych i ambulatoryjnych pacjentów było wrażliwych na piperacylinę, piperacylinę z tazobaktamem, meropenem i ciprofloksacynę. Spośród badanych szczepów najwięcej było opornych na karbenicylinę (69,7%), mezlocylinę (45,5%) i gentamycynę (42,4%). Wykryto 6 (9,1%) szczepów wielolekoopornych. Wśród badanych szczepów 65 (98,5%) produkowało beta-laktamazy indukowane (IBL), 3 (4,5%) wytwarzały metalo-beta-laktamazy (MBL), nie stwierdzono natomiast produkcji beta-laktamaz o rozszerzonym spektrum substratowym (ESBL) hamowanych przez klawulanian.

Abstract.

The aim of this study was to evaluate the drug susceptibility of P. aeruginosa strains and to detect
strains producing inducible beta-lactamases (IBL), extended-spectrum beta-lactamases (ESBL), and metallo-beta-lactamases (MBL). During 6 month (October 2005 – March 2006), 66 strains of P. aeruginosa strains were cultured from clinical specimens obtained from patients of two of hospitals in Siedlce and from patients of outpatient clinics. All the strains were identified in the automatic
ATB (bio Mérieux). The susceptibility of bacteria to antibiotics was tested by standard disc diffusion
method. The majority of strains were susceptible to meropenem (89,4%), piperacillin combined
with tazobactam (84,8%), ciprofloxacin (84,8%) and piperacillin (83,3%). Many of our strains were
resistant to carbenicillin (69,7%), mezlocillin (45,5%), gentamicin (42,4%) and netylmicin (30,3%).
6 strains (9,1%) were multidrug-resistant (MDR). Inducible beta-lactamases were detected with the
use double disc method according to Sanders and Sanders. ESBL-producing strains were detected
with double disc test (DDST) according to Jarlier et al. These strains were identified as ESBL-positive
on the basis of the DDST were also determined using a double disc (DD) test according to Appleton.
Production of metallo-beta-lactamases (MBL) was examined with the use of Etest MBL (AB Biodisk,
Sweden) and the double disc test according to Arakava et al. Sixty-five IBL-producing strains (98,5%
of all strains) and three strains (4,5%) with MBL activity were detected. Strains producing extended
beta-lactamases (ESBL) were not found.

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