Wpływ stymulacji wirusem odry na ekspresję receptorów Toll-like w komórkach PBMC
[Measles virus stimulation effect on the expression of Toll-like receptors in PBMC]

Streszczenie.

W pracy opisano wpływ stymulacji wirusem odry komórek PBMC izolowanych od zdrowych osób szczepionych przeciwko odrze (seropozytywnych i seronegatywnych), na ekspresję receptorów Toll-like. Oceniano ekspresje receptorów TLR2 i TLR4 w odpowiedzi na stymulację ligandami dla tych receptorów (Pam3CSK i LPS) a także w odpowiedzi na stymulację szczepem szczepionkowym (E) i dzikimi (W1 i W2) wirusa odry.

Abstract.

Introduction: Toll-like receptors (TLRs) are an important component of a innate immune system. Stimulation of TLRs, through action with helper T cells, could change Th1/Th2 balance and thus affect adaptive immune response. Receptors TLR2 and TLR4 play important role in immune response to measles virus. The aim of this work was stimulation of the peripheral blood mononuclear cells (PBMC) and determination of Toll-like gene expression in these cells.Methods: PBMCs from 20 healthy donors were stimulated with measles viruses and ligands for TLR2 and TLR4. For examinations the real time RT-PCR (QuantiFast®Assay, Qiagen) was used. The expression of Toll-like receptors was determined on mRNA level, using real-time one step RT-PCR (QuantiFast®Assay, Qiagen) with simultaneous detection of TLR2 and TLR4 genes and housekeeping gene (GAPDH).Results: Virus-specific influence of wild measles virus strains activity on PBMC derived from vaccinated seronegative individuals manifested in higher level of expression of TLR2 and TLR4 genes in compare to the expression of these genes in PBMC of seropositive individuals.Conclusions: Toll-like receptors participate in the development of immune response to measles virus.