Wpływ adhezji i wytwarzania śluzu na zdolność tworzenia biofilmu przez Candida sp.
[Influence of slime production and adhesion of Candida sp. on biofilm formation]

Streszczenie

Ocenę wytwarzania śluzu i zdolności adhezji do polistyrenu przeprowadzono u 50 szczepów Candida sp. izolowanych od pacjentów Kliniki Anestezjologii i Intensywnej Terapii Szpitala Uniwersyteckiego nr 1 im. dr. A. Jurasza w Bydgoszczy, wykorzystując odpowiednio metody według Christensena i wsp. oraz Richardsa. Zdolność tworzenia biofilmu oceniano metodą redukcji TTC na powierzchni dwóch biomateriałów: lateksie silikonowanym i polichlorku winylu. Stwierdzono zależność między wytwarzaniem śluzu i adhezją a zdolnością tworzenia biofilmu.

Abstract

The increase of fungal infections in recent years is connected with the progress in medicine. The vast usage of biomaterials is an inseparable element of contemporary medicine but it also leads to development of infections. Yeast-like fungi Candida albicans are still the main pathogen of candidiasis. The ability to slime production and adhesion to polystyrene of Candida sp. on different surfaces can cause to form biofilm on surfaces of biomaterials used in production of catheters, drains and
prosthesis. The aim of the study was to evaluate the influence of slime production and adhesion to
polystyrene, of Candida sp. on biofilm formation on different biomaterials. 50 strains of Candida sp.
were examined. They isolated from ill to Clinics of Anesthesiology and Intensive Therapy University
Hospital No 1 of dr. A. Jurasza in Bydgoszcz. The ability to slime production was evaluated by Christensen
method in modification Davenport and Branchini methods. The adhesion to polystyrene was evaluated by Richards et el method. The ability to produce biofilm biomaterials by the studied fungi was measured after 72 hours of incubation at 37ºC on different biomaterials. Yeast-like fungi Candida sp. fabricating slime and adhesion forming frequently biofilm on surface researched of biomaterials.
Influence of chosen biological specificity ascertain on the ability to produce biofilm on surfaces of siliconized latex and polyvinylchloride.