SEROLOGICZNE TYPOWANIE I GENOSEROTYPOWANIE PAŁECZEK LISTERIA MONOCYTOGENES IZOLOWANYCH Z PRÓBEK MATERIAŁU KLINICZNEGO, PRÓBEK ŻYWNOŚCI I PRÓBEK ŚRODOWISKOWYCH
[GENOSEROTYPING OF LISTERIA MONOCYTOGENES IN MULTIPLEX PCR]

Streszczenie.

Opisano wyniki typowania szczepów pałeczek Listeria monocytogenes izolowanych z próbek materiału klinicznego, próbek żywności i próbek środowiskowych metodą multiplex PCR umożliwiającą ustalenie przynależności badanego szczepu do określonej genoserogrupy. Stwierdzono, że większość badanych szczepów można było zaliczyć do genoserogrupy I.1 oraz II.2, reprezentujących odpowiednio typy serologiczne 1/2a oraz 1/2b. Szczepy izolowane z próbek żywności najczęściej należały do typu serologicznego 1/2a, natomiast izolowane z próbek materiału klinicznego do 1/2b.

Abstract.

Listeria monocytogenes infection affects people throughout the all world, most often causing gastrointestinal disturbances, less inflammation of the central nervous system (meningitis, meningoencephalitis, absceses), sepsis, endocarditis and dispersed focal inflammation. Listeria monocytogenes
infection in pregnant woman can lead also to premature birth or abortion. Listeriosis is a serious problem because of the high mortality in case of generalized infection, which can reach up to 20%. Listeria strains could be identified by determination of biochemical features – characteristic for each Listeria species. Biochemically identified strains could be serotyped using Seeliger method. Many authors developed a molecular biology-based methods of Listeria serotype determination The
Doumith’s method divide all Listeria monocytogenes serotypes for five genoserogroups. The purpose
of this study was to determine the genoserogroups of Listeria monocytogenes strains and compare
those results with results obtained by classical serotyping by Seeliger method. To this work 90 Listeria strains were used: 75 Listeria monocytogenes strains from clinical, food and environmental samples and 15 reference strains from Pasteur Institute collection and National Institute of Public Health – National Institute of Hygiene collection. All strains were serotyped using liquid stable antisera
for determination of O and H Listeria monocytogenes antigens (Mast Diagnostic, UK). Multiplex PCR for Listeria monocytogenes serovars differentiation were performed according to Doumith et all procedure. The genoserogroup obtained from multiplex PCR of 10 Listeria monocytogenes reference strains were compatible with real serotype. In case of non-Listeria monocytogenes reference strains in
multiplex-PCR, they were identified as non-monocytogenes. In group of 75 non-reference strains
isolated from human, food and environmental sources, 34 belonged to genoserogroup I.1, two strains to genoserogroup I.2, 11 strains to genoserogroup II.1, 21 strains belonged to genoserogroup II.2 and 9 strains to genoserogroup III.