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Medycyna Doświadczalna i Mikrobiologia 2017, 69(1): 27-34

Ocena przydatności opracowanego we własnym zakresie lateksowego testu aglutynacyjnego do poszukiwania przeciwciał dla antygenów pałeczek Yersinia enterocolitica i Yersinia pseudotuberculosis u osób z jersiniozą
[Development and evaluation of a latex agglutination test for the detection of antibodies to Yersinia enterocolitica and Yersinia pseudotuberculosis in humans with yersiniosis]

Waldemar Rastawicki, Anna Chróst, Kornelia Gielarowiec

STRESZCZENIE

Oceniono przydatność opracowanego we własnym zakresie lateksowego testu aglutynacyjnego do poszukiwania przeciwciał dla antygenów pałeczek Yersinia enterocolitica i Yersinia pseudotuberculosis w surowicy osób z jersiniozą. Wchodzący w skład zestawu odczynnik z zawiesiną pałeczek Yersinia zapewnia wysoką czułość (97,0%), natomiast odczynnik z zawiesiną białek wydzielniczych Yop wysoką swoistość (96,4%) testu w stosunku do odczynu

ELISA. Opracowany test lateksowy, charakteryzujący się prostotą i szybkością wykonania może być przydatny we wstępnej, różnicującej serodiagnostyce jersiniozy.

ABSTRACT

Introduction: The aim of the study was a molecular characterisation of Staphylococcus aureus strains isolated from surgical site infections (SSIs) from patients in southern Poland, undergoing different surgical procedures, together with evaluation of the prevalence of antimicrobial resistance and the presence of virulence factors.

Materials and Methods: In this laboratory-based, multicentre study, non-repetitive 162 samples from SSI were collected from hospitalised patients (12 hospitals, n=139) or outpatients (n=23) in southern Poland between January 1 and December 31, 2013. In all S.aureus isolates, we investigated antimicrobial susceptibility, the presence of selected virulence genes (lukE, pvl, tsst-1 and eta), and also conducted spa typing.

Results: Patients with SSI had a median age of 61 years; 54.9% were male. Prevalence of MRSA (29 strains, 17.9%) SSI per surgery type was 8.7% in orthopaedic, 17.7% in general and 42.9% in vascular surgery. Over 20% of strains were resistant for erythromycin (27.2%), clindamycin (23.5%) and ciprofloxacin (23.5%). No resistance was found for linezolid, glycopeptides or tigecycline. Gene of leukocidin (lukE) was the most frequently found gene. Spa typing identified 10 spa types; the two dominant types were t003 (41.4%) and t138 (17.2%).

Conclusions: The results show that after vascular surgery, there was an unexpectedly high prevalence of MRSA in SSIs in southern Poland. Conversely, the prevalence of MRSA was unexpectedly low following orthopaedics procedures. The surprisingly observation was the low virulence of the S. aureus strains among older patients.

Keywords: Staphylococcus aureus, surgical site infections, Poland, MRSA

 

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